Figure 5. TALEN-mediated genome editing for correction of RDEB.
(a) RDEB patient suffering from chronic blistering. Panel a reprinted with permission from Elsevier (Petrof et al., 2015). (b) Confocal image of the epidermal-dermal junction in noncorrected RDEB cells, in which collagen VIIA is not detected. (c) Confocal image of the epidermal-dermal junction in gene-corrected RDEB cells, in which collagen VIIA is detected in the red channel. Panels b and c reprinted with permission from Macmillan Publishers Ltd: Molecular Therapy (Osborn et al., 2013). RDEB, recessive dystrophic epidermolysis bullosa; TALEN, transcription activator-like effector nuclease.