Fig. 3.

Co-transfection experiments, involving pair-wise application of two encoding plasmid DNAs. Equal amounts (by weight) of pDNA encoding for the respective reporter proteins under the control of the intermediate early CMV promoter were used, with the total amount of pDNA equaling that used in the experiments involving only one reporter protein under otherwise identical experimental conditions. Expression of EGFP (diagonal top right lines), YFP (diagonal top left lines) and RFP (horizontal lines) under the control of the intermediate early CMV promoter was quantified 48 h post transfection. Indicated are bichromatic cells expressing both proteins and monochromatic cells expressing only one of the proteins. Transfected cells population was further divided into: low producers (white, fluorescence signal between 1 and 10 a.u.), middle producers (grey, fluorescence signal between 10 and 100 a.u.), and high producers (dark grey, fluorescence signal >100 a.u.).