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(a) The 8-17 DNAzyme with uncleavable substrate strand (17DDS) and the terminal FRET pair modifications and their structures. (b) An example of spectral fitting with trifluorophore-modified DNAzyme. (c) Proposed two-step mechanism of DNAzyme folding in the presence of Zn2+ ions. Figures are adapted with permission from ref 115.
