Figure 4.
A scheme of conserved sequences of the 8-17 DNAzyme revealed by mutational analysis. Mutations of the bases shown in black to any other natural base resulted in >100-fold decrease in activity. Removal of the functional groups highlighted in gray circles caused >100-fold decrease in activity. Residues shown in gray are those whose replacement by modified bases caused >10-fold decrease in activity. Residues shown in white were more tolerant to mutagenesis. Figure adapted with permission from ref 86.