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. 2016 Jan 8;2(3):369–383. doi: 10.1016/j.jcmgh.2015.12.006

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Lysozyme secretion in ZnT2ko mice. (A) Representative immunoblot for lysozyme (and β-actin loading control) in harvested ileal segments from wt and ZnT2ko mice after perfusion with saline. (B) Lysozyme abundance in the ilea of wt and ZnT2ko mice. ZnT2ko mice had significantly less lysozyme overall (n = 6/genotype, **P < .01). (C) Lysozyme release into the small intestinal lumen at baseline. In unstimulated ileum, the ZnT2ko released relatively greater lysozyme in comparison with the wt (n = 6/genotype, *P < .05). (D) Ratios of dimeric to monomeric lysozyme were increased significantly in the ilea of ZnT2ko mice (n = 6/genotype, *P < .05). Immunoblots were performed in duplicate.