Fig. 5.

Cytotoxicity of permeability treatments on hCMEC/D3s. The pharmacological treatments used were assessed for any cytotoxic potential using an MTT assay with confluent monolayers of hCMEC/D3 cells in 96 well plates, grown in permeability medium as described in Section 4.3. The results are expressed as percentage viability±SEM and compared to control untreated cells, which were incubated in permeability medium alone. TNF-α and IFN-γ were used together at 5 ng/ml each. N=3 plates, with 6 replicates per plate.