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Formation of ROS in hCMEC/D3 cells. hCMEC/D3 cells were treated with increasing doses of ADMA for 24 h before assessment of ROS levels with DHE. As a positive control, some wells were treated with 300 µM H2O2 alone for 30 min before measuring ROS levels. ADMA treatment had no significant effect on ROS levels in hCMEC/D3 cells, while exposure to H2O2 significantly increased ROS in these cells (one way ANOVA,*p<0.05).
