Table 3. Splice isoforms.
| SPLICE ISOFORMS | % | |
|---|---|---|
| US1 | double-spliced | 96.6 |
| non-spliced | 1.4 | |
| single spliced (intron 1) | 1.2 | |
| single spliced (intron 2) | 0.8 | |
| UL15 | spliced | 53.3 |
| non-spliced | 46.7 | |
| EP0 | non-spliced | 77.6 |
| spliced (intron 1) | 1.7 | |
| spliced (intron 2) | 20.7 | |
PacBio sequencing revealed that all four splicing combinations of the US1 transcripts are expressed in the lytic cycle of the virus. We calculated the relative proportions of the splice variants in the known spliced transcripts, including US1, EP0 and UL15.