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. 2016 Sep 29;11(9):e0163670. doi: 10.1371/journal.pone.0163670

Fig 3. Influence of PEG on protein production in cell-free systems based on translationally active CHO lysate.

Fig 3

Two concentrations (1%, 2%) of different PEG molecules (3350, 5000, 20000) were analyzed in cell-free protein synthesis reactions using pIX3.0-CRPV(GCT)-eYFP plasmid template. Translation reactions without the addition of PEG but with supplementation of 3 U/μl (increased concentration) and 1 U/μl (standard concentration) T7 RNA polymerase served as control reactions A. Fluorescence signals of synthesized eYFP proteins were detected by fluorescence imaging on μ-Ibidi slides using the Typhoon Trio Plus Imager. B. Quantification of fluorescence signals was accomplished by using Image Quant TL Array analysis software. Error bars show standard deviations calculated from triplicate analysis.