Table 2. “Difficult-to-express” proteins synthesized in the CHO lysate based cell-free system.

Name of protein	Gene name (Abbreviation)	Type of protein	Organism	Molecular mass [kDa]	Identifier (Uniprot)
pH-gated potassium channel KcsA	KcsA	Potassium channel	Streptomyces lividans	17.6	P0A334
Voltage-gated potassium uptake system KvaP	KvAP	Voltage-gated potassium channel complex	Shewanella oneidensis	30.8	Q8EAX3
Transient receptor potential cation channel subfamily V member 1	TRPVI	Calcium channel	Homo sapiens	94.9	Q8NER1
Epidermal growth factor receptor	EGFR	Transmembrane receptor protein tyrosine kinase	Homo sapiens	134.2	P00533
Potassium voltage-gated channel subfamily H member 2	hERG	Voltage gated Potassium channels	Homo sapiens	126.6	Q12809
Mu-type opioid receptor	OPMR1	G-protein coupled receptor	Homo sapiens	44.7	P35372
Channelrhodopsin-2	ChRh2	Ion channel	Volvox carteri f. nagariensis	77.8	B4Y105
Beta-2 adrenergic receptor	B2AR	G-protein coupled receptor	Homo sapiens	46.4	P07550