Fig 8. Analysis of the turnover rate of proteins found in the analysis of the yeast peptides.

Protein half-life data were reported by Christiano et al [32] and were determined by growing yeast in heavy [13C6/15N2] L-lysine for many generations, then growing in light lysine for various lengths of time and measuring the ratio of heavy/light proteins using mass spectrometry-based proteomics. The half-life was capped by Christiano et al at 100 hours. Out of 75 proteins found in our peptidomics analysis, 73 corresponded to proteins detected by Christiano et al. The relative half life (log scale) for these 73 proteins and for all 3772 proteins reported by Christiano are plotted as a percentile of the half-life for each group of proteins.