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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2016 May 7;43:18–23. doi: 10.1016/j.dnarep.2016.04.004

Fig. 5.

Fig. 5

Effects of SCR7 derivatives on double-strand ligation by LigIIIα/XRCC1 and LigIV/XRCC4. (A) Schematic structure of the DNA substrate for double-strand ligation. The 5′ terminus is 32P-phosphorylated. (B) The DNA substrate (0.2 pmol) was incubated with either LigIIIα/XRCC1 (0.2 pmol) or LigIV/XRCC4 (1 pmol) in the absence or presence of the indicated concentrations of SCR7 derivatives prior to electrophoresis in 7 M urea-containing 12.5% polyacrylamide gels. The products resulting from ligation of both strands (ds-ligated) and only one strand (ss-ligated) as well as the original substrate (unligated) are indicated. (C) The results of the inter-molecular ligation assays are shown graphically. LigIIIα/XRCC1 (open symbols) and LigIV/XRCC4 (closed symbols) incubated with either SCR7-R (circles) or SCR7-X (squares). Ligation efficiency was calculated by setting the reactions with no inhibitor as 100%. Data are the mean ± standard deviation of three independent experiments.