Table 3. tested and recommended products for working with Qdots.

	Original	Vivid	Notes
PBS	Quenching	Quenching	avoid (even if manufacturer’s protocol still recommends all solutions in PBS)
TBS	photobleaching	Quenching & photobleaching	avoid
Bond Wash	photobleaching	Quenching & photobleaching	avoid
Tween @ 0.1%	quenching	quenching	“stable up to 0.5% “in manufacturer information
			avoid
BSA	Provides some protection from quenching (in Tris)		Test further if needed
Qdot diluant	Good—Moderate quenching in solution		Used in multiplex
Borate buffer	Moderate quenching in solution		Use with caution
TRIS	quenching		Use with caution to dilute BKRA if cost needs to be kept low
BKRA	stable		Used in multiplex
SFB	Fairly stable		Used in multiplex
Qdots
525	Stable		Original Qdot–very stable and reliable in most buffer tested
565	Stable		Original Qdot–very stable and reliable in most buffer tested
585V	Least stable in all buffer tested including BKRA		Avoid if possible Control for stability at all times
605V	Average stability		Control for stability at all times
625V	Good stability		Control for stability
655V	Average stability		Control for stability at all times
705V	Good stability overall		Control for stability
Cytoseal	Good stability for 1 to 2 weeks		Control for loss of fluorescence with time if quantitative analysis over more than 1 day
Ecomount	Good stability for at least 2 weeks		recommended