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. 2016 Sep 29;11(9):e0163777. doi: 10.1371/journal.pone.0163777

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© 2016 Crowley et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Relative sFRP2 mRNA expression by qPCR in (A) intact human SC and OM AT, (B) in isolated and differentiated human adipocytes and in (C) adipose stromal cells (ASCs). (D) Western immunoblot of sFRP2 shows band intensity (area) from adipose tissue conditioned media (ATCM) from 5 paired SC and OM explants after 48h incubation. An insert is a representative Western blot from 2 paired samples. ATCM was obtained by incubating the same amount of AT in 5ml media volume, n = 5 (D). *<0.05, **<0.01, ***<0.001.