Table 1. Mouse bioassay of hamster scrapie brain homogenates after treatment with disinfectants.

Treatment	Dilution of scrapie brain homogenate after treatment a
	10−3	10−4	10−5	10−6	10−7	10−8	10−9	10−10	Titer b
Saline	nt	4/4 c [56]	4/4 [66]	4/4 [66.8]	4/4 [77.3]	2/4 [107.5]	1/4 [101]	0/4	6.75
HOCl	0/4 d	0/4	0/4	0/4	0/4 d	0/4	nt	nt	≤ 1
NaOH	nt	nt	0/4	0/4	0/4	0/4	nt	nt	≤ 3
Bleach	nt	nt	0/4 d	0/4	0/4 d	0/4	nt	nt	≤ 3
LpH	nt	nt	0/4 d	0/4	0/4	0/4	0/4 d	nt	≤ 3

^a Scrapie brain homogenates (10⁻³ dilution) were exposed to different disinfectants or saline for 1 h at a 1:100 (v:v) ratio. Solutions were then further diluted for bioassay in tg7 mice. Each recipient mouse received 30μl of inoculum.

^b The calculated titer reported is the logLD₅₀/mg of tissue

^c The numerator is the number of scrapie-positive mice, and the denominator is the number of mice inoculated. For groups with positive mice the average scrapie incubation period is provided. Mice that did not develop disease were observed for 200 days post inoculation (dpi).

nt = Not tested (see methods for explanation)

^d Indicates a mouse/mice in this group was/were euthanized between 73–186 dpi due to dermatitis, intercurrent disease or were found deceased. Brains from these mice were screened for TSE infection by RT-QuIC for prion seeding activity and by Western blot for protease-resistant PrP. No mice in these groups were diagnosed with scrapie.