Table 2. Bioassay of scrapie-coated wires after treatment with disinfectants.
| Treatment | Dilution of 263K ScBH | |||||||
|---|---|---|---|---|---|---|---|---|
| 10−3 | 10−4 | 10−5 | 10−6 | 10−7 | 10−8 | 10−9 | 10−10 | |
| None | 4/4 b [99±18] | 4/4 [90±10] | 4/4 [90±4] | 4/4 [144±7] | 0/3 | 0/4 | 0/4 | 0/4 |
| Saline | 6/7 [96±14] | nt | nt | nt | nt | nt | nt | nt |
| HOCl | 0/7 | nt | nt | nt | nt | nt | nt | nt |
| NaOH | 1/8 [137] | nt | nt | nt | nt | nt | nt | nt |
| Bleach | 0/8 | nt | nt | nt | nt | nt | nt | nt |
| LpH | 0/8 | nt | nt | nt | nt | nt | nt | nt |
a Steel wires were exposed to hamster 263K ScBH, then washed, dried and either untreated (None) or treated by immersion in saline or the designated disinfectants for 1 h. Following treatment wires were removed and allowed to dry. Each mouse was implanted intracerebrally with a single 3–4 mm wire.
b The numerator is the number of scrapie-positive mice, and the denominator is the number of mice implanted. For groups with positive mice the average scrapie incubation period +/- SD is provided in brackets. Mice that did not develop disease were observed for 200 d.