Fig 3. All possible sub-variants detected in the Illumina RNA-Seq data.

Considering the three major 5’ ends and two major 3’ ends, there are 21 “sub-variants” across 8 “regions”, which can be combined to form 648 different possible combinations. Based on coverage in the Illumina and PacBio data, we narrowed this down to 9 “major” sub-variants that appear to be expressed in the pineal gland in substantial quantities. There are 24 possible combinations of these 9 major sub-variants.