Table 3. Distribution of “reads of insert” (ROI) from the SMRT sequencing.

		# ROI	% ROI
Matches known, valid primer pair (“Full-Length”)		257,018	62.5%
Non Full length		153,963	37.5%
	No primer match	79,413	19.3%
	Matches known primer on one end	69,817	17.0%
Aligns uniquely to genome		305,675	74.4%
	Aligns to location other than Ttc8	1,550	0.4%
	Aligns to Ttc8 and is full-length	242,045	58.9%
Full-length and matches predicted isoform		180,479	43.9%
	Perfect full-length match to major isoform	56,977	13.9%
	Alignment match to major isoform	97,677	23.8%
	Matches major isoform with 8-9b intron retention	24,064	5.9%
	Matches isoform with minor splicing variant	1,761	0.4%
Total Reads of Insert		410,981	100.0%