Fig 8. Treatment of 2D cell culture by a BACE1 inhibitor.

2D neurons differentiated from five AD patients’ iPSC lines were treated with increasing concentrations of BACE1 inhibitor (BI, 0.1, 0.5 and 1 μM; blue) or DMSO as control (grey) for two days. Conditioned media were collected and Aß 40 and 42 were quantified by ELISA. Both Aß 40 (A) and 42 (B) levels were significantly reduced in all lines from 5 AD subjects after the treatment with the BACE inhibitor at all doses tested. Levels of Aβ42 were undetectable in 1 μM BI-treated neurons derived from AD5. The graph shows Mean±SEM; * represents p< 0.05, comparison of inhibitor vs. DMSO.