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. 2016 Sep 29;11(9):e0163337. doi: 10.1371/journal.pone.0163337

Fig 3. ATR volatile oil, α-asarone or β-asarone, potentiates NGF-induced expression of neurofilaments.

Fig 3

(A) Cultures were co-treated with ATR volatile oil, α-asarone or β-asarone, at 30 μg/mL with NGF (0.5 ng/mL) for 48 hours. NGF at 50 ng/mL served as a control. The cell lysates were collected to determine the expression of NF68, NF160 and NF200. GAPDH served as loading control. (B) Quantification from the blots by a densitometer was shown. Values were expressed as the fold of change (x Basal) against the control (no treatment; set as 1), and in Mean ± SEM, n = 4, each with triplicate samples.