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. 2016 Sep 29;11(9):e0163337. doi: 10.1371/journal.pone.0163337

Fig 4. Inhibition of PKA suppresses neurofilament expression, induced by ATR volatile oil, α-asarone or β-asarone, in cultured PC12 cells.

Fig 4

(A) Cultured PC12 cells were pre-treated with or without PKA inhibitor, H89 (5 μM) or KT5720 (1 μM), for 3 hours, and then treated with ATR volatile oil, α-asarone, β-asarone, at 30 μg/ mL, or NGF at 50 ng/mL, for 48 hours. The cell lysates were collected to determine the expressions of NF68, NF160, and NF200. GAPDH served as a loading control. (B) Quantification from the blots by a densitometer was shown. Values were expressed as the fold of change (x Basal) against the control (no treatment; set as 1), and in Mean ± SEM, n = 4, each with triplicate samples. * p < 0.05; ** p < 0.01; *** p < 0.001.