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. 2016 Sep 29;11(9):e0163337. doi: 10.1371/journal.pone.0163337

Fig 7. Different combination ratios of α-asarone and β-asarone on increasing NF promoter activity.

Fig 7

(A) The concentration of α-asarone of set at 5 μg/mL, and the concentration of β-asarone was varied (0.625–40 μg/mL) to form different ratios from 8:1 to 1:8. A mixture of asarone in defined ratio/concentration was applied onto pNF68/200-Luc transfected PC12 cells. The luciferase activity was determined after 48 hours. (B) The concentration of β-asarone of set at 5 μg/mL, and the concentration of α-asarone was varied (0.625–40 μg/mL) to form different ratios from 8:1 to 1:8. The cell treatment was as in (A). (C) Combination of α-asarone and β-asarone in 1:4 ratio, α-asarone or β-asarone were applied onto pNF68-Luc transfected PC12 cells in different concentrations. The luciferase activity of each sample was determined after 48 hours. (D) The treatment was as in (C) onto pNF200-Luc transfected PC12 cells. Values are expressed as percentage of increase as compared to control (without drug treatment), and in Mean ± SEM, n = 4, each with triplicate samples. * p < 0.05; ** p < 0.01; *** p < 0.001.