Skip to main content
. 2016 Sep 29;11(9):e0163302. doi: 10.1371/journal.pone.0163302

Fig 4. Enrichment of glycoproteins from T. cruzi epimastigote using TL and GSLII affinity chromatography.

Fig 4

T.cruzi epimastigote proteins were fractionated by detergent extraction into CHAPS and CHAPS + Triton X-114 fractions. These fractions were loaded either onto agarose-coupled TL or GSLII beads columns and left overnight at 4°C on a rotating device. Whole cell extracts, columns flow-through and eluates were then separated on NuPAGE gels (4–12%) and proteins were revealed by SafeStain blue staining.