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. 2016 Sep 29;11(9):e0163784. doi: 10.1371/journal.pone.0163784

Fig 1. Cannulae placement verification.

Fig 1

Left, A schematic of the brain section from Paxinos and Watson [40]. Right, A representative photomicrograph of cresyl violet-stained brain slice (75 μm-thick) used to confirm cannulae placements in the NAc shell. Dotted circle (1 mm diameter) represents the maximal diffusion area for a 0.5 μl solution volume [41]. Black arrow–tip of the guide cannula. White arrow–tip of the microinjector. White arrowhead–lateral ventricle. AC–anterior commissure. Scale bar, 0.5 mm.