Fig 1. Effect of chronic (7 days) treatment of BaP, NPh, and Phe on mRNA and protein expression of CYP1A1 (A-B) and CYP3A4 (C-D) in U937 cells.

The U937 cells were treated with 100 nM BaP, 100 nM NPh, and 100 nM Phe for seven days. The mRNA fold expressions were calculated using qRT-PCR and normalized with control (DMSO treated cells) whose expression was set at 1-fold. GAPDH was used as an endogenous control. The protein fold expressions were quantified by Western blot and normalized with control that was set to 1-fold at every time point. Blots are representative of at least three independent experiments. The data are presented as a mean ± SEM of three independent experiments. *represents p ≤ 0.05, compared with the control group.