Fig 6. Cell viability (A), Reactive Oxygen Species (ROS) level (B) and mRNA expression level of CYP1A1 (C) and CYP3A4 (D) upon chronic treatment by BaP in primary macrophages.

The differentiated macrophages obtained from healthy donors were treated with BaP (100 nM) for 6 days. Cell viability and ROS were calculated were calculated and normalized with control that was set as 100%. The mRNA expression of treated cells were normalized with control (DMSO treated cells) whose expression was set at 1-fold. The results for cell viability were confirmed using 3 different donors. Due to inadequate number of primary cells, ROS and CYPs expression level were examined in only one donor. The data is presented as mean ± SEM of the three replicates for ROS and two replicates for CYP expression levels. * represents p ≤ 0.05, compared with the control group.