Figure 9.

Netrin-1 is positively selected for translation during UPR in mice. C57BL6 mice were treated with PBS or 1 mg/kg Tu and killed 24 hours after treatment. Livers were snap-frozen, disrupted in dry ice, and then resuspended in polysome lysis buffer. Lysates were subjected to sucrose gradient fractionation followed by qRT-PCR to quantify gus, β-actin, netrin-1, c-myb, c-myc, and ATF4 V1 mRNAs. (A) Distribution of mRNAs across sucrose gradients. Bar graph represents RNA fractions processed for β-actin and netrin-1 mRNA quantification (mean + sem, n = 5 [PBS], n = 8 [Tu] for the entire figure). Agarose gel electrophoreses reflects ribosomal RNA distribution in the gradient. (B) The evolution of the association of indicated mRNAs with polysomes was determined as the percentage difference of polysome-associated mRNA signals in Tu samples vs mock samples in each polysome profile. See also Supplementary Figure 5.