Supplementary Figure 3.

Netrin-1 mRNA association with translational units is unchanged during UPR in Huh7.5 cells. Huh7.5 cells were treated with 2.5 mM DTT for 4 hours of left untreated (Mock). Cell lysates were subjected to sucrose gradient fractionation followed by RT-qPCR for quantification of β-actin, netrin-1, l-myb, c-myb c-myc and ATF4 V1 mRNA. (A) Distribution of mRNAs in the sucrose gradient. Agarose gel electrophoreses show ribosomal RNA distribution across sucrose gradient. Bar graph represents RNA fractions processed for β-actin and netrin-1 mRNA quantification. (Mean + sem). (B) The evolution of the association of indicated mRNAs with polysomes was determined as percentage difference of polysome-associated mRNA signals in DTT samples vs mock samples in each polysome profile.