Supplementary Figure 5.

Differential association of Netrin-1 related transcript with translational units during UPR in mice. C57BL6 mice were treated with PBS or 1mg/kg Tunicamycin (Tu) and sacrificed 24 hours post-treatment. Livers were snap-frozen, disrupted in dry ice and then resuspended in polysome lysis buffer. Lysates were subjected to sucrose gradient fractionation followed by RT-qPCR to quantify gus, c-myb c-myc and ATF4 V1 mRNAs. Figure shows distribution of mRNAs across sucrose gradients. Bar graph represents RNA fractions processed for β-actin and netrin-1 mRNA quantification ((Mean + sem, n = 5 (PBS), n = 8 (Tu)). Agarose gel electrophoreses reflects ribosomal RNA distribution in the sucrose gradient. (Representative result, n + 5 (PBS), n + 8 (Tu)).