Supplementary Table 3.
Other Primers
| Gene name | Genbank accession number | Primer sequences | PCR conditions | Amplicon length, bp |
|---|---|---|---|---|
| Netrin-1 (5’UTR 1-294) | NM_004822 | Forward: (T7p5’UTR_Netrin-1) TAATACGACTCACTATAGCTTCGGGGGCGAGC; reverse: (cas-as5’UTR_Netrin-1) GAACCGGACCGAAGCCCGATTTGGATCCGGCG AACCGGATCGACCTTGCCGAAGGCCGCAT |
Denaturation, 95°C; annealing, 65°C (qPCR with 10% DMSO) |
294 |
| / | / | Forward: (primer Std) GAACCGGACCGAAGCCCG | / | / |
| Fluc | / | Forward: TGTCCACCTCGATATGTGCATC | / | / |
/ signify that there is no gene name, PCR conditions and amplicon length for these primers. They were used as labeled probes. DMSO, dimethyl sulfoxide.