TABLE 1:

Effects of EB1 and tau on microtubule dynamics.

	Growth rate (μm/min)	Shortening rate (μm/min)	Catastrophe frequency (events/min)	Rescue frequency (events/min)
Control	1.53 ± 0.25	31.78 ± 4.73	0.149 ± 0.014	0
	n = 119	n = 102	n = 103
GFP-EB1 (75 nM)	1.91 ± 0.33	32.66 ± 5.62	0.232 ± 0.031	0
	n = 59	n = 56	n = 56
tau (15 nM)	1.75 ± 0.28	21.09 ± 4.36	0.039 ± 0.008	1.66 ± 0.35
	n = 54	n = 22	n = 20	n = 22
tau (35 nM)	1.70 ± 0.27	20.92 ± 4.99	0.018 ± 0.005	1.21 ± 0.38
	n = 45	n = 12	n = 12	n = 10
tau (75 nM)	2.06 ± 0.27	19.32 ± 5.52	0.005 ± 0.0025	1.58 ± 0.79
	n = 51	n = 4	n = 4	n = 4
GFP-EB1 + 15 nM tau	1.88 ± 0.35	26.01 ± 6.09	0.153 ± 0.015	1.75 ± 0.20
	n = 125	n = 104	n = 97	n = 73
GFP-EB1 + 35 nM tau	1.76 ± 0.34	22.18 ± 4.35	0.064 ± 0.010	2.61 ± 0.43
	n = 64	n = 37	n = 37	n = 36
GFP-EB1 + 75 nM tau	1.93 ± 0.23	18.73 ± 3.59	0.004 ± 0.002	1.36 ± 0.68
	n = 42	n = 4	n = 4	n = 4

Dynamic parameters were measured for microtubules assembled with tubulin (12 μM) in the absence or presence of the indicated proteins. The total times of measurements (growing and shrinkage phases) were 723.80, 261.92, 514.74, 666.96, 742.74, 674.38, 583.67, and 850.78 min for tubulin alone, 75 nM GFP-EB1, 15 nM tau, 35 nM tau, 75 nM tau, 75 nM GFP-EB1 + 15 nM tau, 75 nM GFP-EB1 + 35 nM tau, and 75 nM GFP-EB1 + 75 nM tau, respectively. n, number of events measured for each condition. Values represent mean ± SD.