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. 2016 Oct 1;27(19):2965–2979. doi: 10.1091/mbc.E15-09-0634

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© 2016 Kelpsch et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Nuclear GFP-Actin rods are stable. (A–D) Representative example of FRAP experiments. Single-slice confocal images of GFP-Actin 5C (UAS GFP-Actin 5C; mat3Gal4) during FRAP time course showing prebleach, postbleach, middle, and endpoint. Scale bar, 10 μm. (E) Average FRAP recovery curve. Relative fluorescence intensity over time for nine different nuclei from five follicles. Error bars, SD. The nuclear GFP-Actin rods are stable, as the bleached region fails to recover.