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NADPH-diaphorase activity. Quantification of neurons in the myenteric plexus from diabetic NOD mice with delayed gastric emptying treated with vehicle or 1 μg IL10 twice a day. Positively labeled neurons were counted from 7 high-power fields using a 20× objective from each tissue. A minimum of 174 neurons were counted from each tissue. There was no significant difference in the number of positively labeled neurons between the 2 groups (means ± SEM, t test).
