Figure 4. AKHR⁺ neurons are required for starvation-induced hyperactivity.

(a, c) The expression of membrane-bound GFP (mCD8GFP) in AKHR⁺neurons in the anterior (a) and the posterior (c) part of the fly brain. The dashed box outlines the region of SEZ. (b, d) An enlarged image of the SEZ region seen in a and c. Note the cell bodies located in the ventrolateral side of the SEZ (arrowheads) and their Y-shaped projections (arrows) in b. Green: GFP. Magenta: nc82. Scale bars represent 20 μm in a–d. (e–f) Midline crossing activity of indicated genotypes assayed in the presence of 5% sucrose ('+Sucrose') or 2% agar ('-Sucrose') (n = 60–75). (g) Average daily midline crossing activity of flies assayed in e–f. (h) Volume of 800 mM sucrose consumed in a meal by indicated genotypes fed ad libitum with 5% sucrose, or starved for 24 hr using the MAFE assay (n = 18–40). (i–j) Number of feeding bouts (i) and total duration of feeding time (j) during 1 hr recording in the FLIC assay (n = 20–24). Error bars represent SEM. NS, p>0.05; ***p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.15693.009

Figure 4—figure supplement 1. AKHR expressed in Gr5a⁺ gustatory sensory neurons is not required for starvation-induced hyperactivity.

(a–b) Midline crossing activity of indicated genotypes assayed in the presence of 5% sucrose ('+Sucrose') or 2% agar ('-Sucrose') (n = 46–64). (c) Average daily midline crossing activity of flies assayed in a–b. ***p<0.001.

DOI: http://dx.doi.org/10.7554/eLife.15693.010