Figure 7. HSM-AD analysis of GNS@SiO2.
(a) Classification of unstained control tissues yields negligible false-positive detection for GNS@SiO2 (green), which exhibit a peak plasmonic resonance of ~550 nm. (b,c) Intravenously-administered GNS@SiO2 accumulate in the Kupffer cells of the liver and the marginal zone of the spleen within 2 hr (b) and persist up to 24 hr (c).
DOI: http://dx.doi.org/10.7554/eLife.16352.038
Figure 7—source data 1. Data for GNS@SiO2 uptake in organs.
elife-16352-fig7-data1.txt (8.8KB, txt)
DOI: 10.7554/eLife.16352.039
Figure 7—figure supplement 1. Structural and spectral characterization of GNS@SiO2.
(a) TEM of GNS@SiO2. (b) Vis-NIR absorbance spectrum of GNS@SiO2 in water (SPR ~550 nm). (c) Spectral library clusters identified from training on images of unstained tissues (in CytoSeal) resected from mice injected with GNS@SiO2. The target number of clusters was set to three rather than five (used for LGNR detection) due to the absence of H&E staining. Cluster 2 (green) corresponds to the GNS@SiO2 and exhibits a red-shifted plasmonic peak relative to particles in water, as expected due to the difference in refractive environment.
Figure 7—figure supplement 2. Detail of GNS@SiO2 in spleen.
HSM-AD reveals that GNS@SiO2 accumulate mostly in the marginal zone tissue between red and white pulp. The approximate boundaries between red pulp and white pulp follicles are marked by dashed white lines.
Figure 7—figure supplement 3. HSM-AD quantification of GNS@SiO2 uptake in liver and spleen tissue.
Relative GNS@SiO2 uptake was measured using the positive ratio approach described in the methods section. All values represent the mean GNS@SiO2 uptake from 4 FOVs for each injection/tissue combination. Error bars represent standard error of the mean (s.e.m.).
Figure 7—figure supplement 4. Inductively-Coupled Plasma Mass Spectrometry (ICP-MS) quantification of GNS@SiO2 uptake in liver and spleen tissue.
Quantitative measurements of atomic gold present in tissue samples prepared through microwave digestion. Counts are given in parts per billion (ppb) of Au.
Figure 7—figure supplement 5. Additional HSM-AD images of GNS@SiO2 uptake in liver tissue.
Quantitative data from these FOVs were used to produce the bar graph in Figure 7—figure supplement 3.
Figure 7—figure supplement 6. Additional HSM-AD images of GNS@SiO2 uptake in spleen tissue.
Quantitative data from these FOVs were used to produce the bar graph in Figure 7—figure supplement 3.