Figure 2.
Effect of PRO20 on urine concentrating capability in rats. PRO20 or vehicle was administered to Sprague–Dawley rats via a catheter chronically implanted in the kidney for 7 days followed by WD for 48 hours. The second 24-hour urine was analyzed. (A) Urine volume after WD (n=8 rats per group). (B) Urine osmolality after WD (n=8 rats per group). (C and D) Immunoblotting analysis of AQP2 was done in the cortex and inner medulla (n=8 rats per group). *P<0.05 versus control; #P<0.05 versus WD alone. (E) ELISA measurement of urinary AVP (n=6 rats per group). Data are means±SEMs. CTR, control.