Figure 3.
Effect of EP4 antagonism on urine concentrating capability in rats. Sprague–Dawley rats were pretreated with vehicle or ONOant for 7 days and water deprived for 48 hours with or without ONOant treatment or had free access to water. ONOant was mixed in chow diet at 0.2 mg/kg per day. (A) Urine volume during 48 hours of WD (n=8 rats per group). (B) Urine osmolality during 48 hours of WD (n=8 rats per group). (C) Immunoblotting analysis of AQP2 in the cortex (n=6 rats per group). (D) Immunoblotting analysis of AQP2 in the inner medulla (n=6 rats per group). Data are means±SEMs. CTR, control. *P<0.05 versus control; #P<0.05 versus WD alone.