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Validation of AQP2-Cre activity in intercalated cells. A–C show colocalization of YFP and AQP2 in mice doubly heterozygous for ROSA26-YFP and AQP2-Cre. D–F show colocalization of YFP and AE1 at low magnification. G–I show colocalization of YFP and AE1 at high magnification. Shown are representative photomicrographs from at least three animals per group. All images were derived from immunofluorescence of the renal medulla. IC, intercalated cell.
