Figure 10.

Inhibition of Orai1 Ca²⁺ channel by shRNA prevented increase of fibronectin and collagen IV induced by TGF‑β1 in HMCs. (A, B) Orai1 inhibition by shRNA increased expression of fibronectin and collagen IV in cultured HMCs, but suppressed increase of fibronectin and collagen IV expression induced by TGF‑β1. Values are mean±SEM, n=6 in each group. *P≤0.05 or **P≤0.01 versus Ad‑GFP–shRNA; ^#P≤0.05 or ^##P≤0.01 versus TGF‑β1+Ad‑GFP–shRNA. (C, D) Abundance of Orai1 protein expression in HK2 cells were more than that in mesangial cells. Values are mean±SEM, n=6 in each group. *P≤0.05 versus HMCs.