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. 2016 Mar 3;27(10):3063–3078. doi: 10.1681/ASN.2015080889

Figure 2.

Figure 2.

HFD was associated with increased expression of Orai1 in the kidneys of ApoE–/– mice. (A–D) Orai1 protein and (E) mRNA expression were significantly upregulated in the kidney cortex of ApoE–/– mice fed with HFD compared with controls by immunoblotting (A, B), immunohistochemistry (C, D), and quantitative real-time PCR (E). Values are means±SEM, n=4–6 in each group. *P≤0.05 versus normal diet. Ad‑Orai1–shRNA successfully inhibited (F) protein abundance and (G) mRNA levels of Orai1 in the kidney cortex of ApoE–/– mice fed with HFD. Values are means±SEM, n=10 in each group. **P≤0.01 versus HFD+Ad‑GFP–shRNA.