Figure 4.

Inhibition of Orai1 Ca²⁺ channel by shRNA or SKF96365 prevented increased protein abundance of profibrotic factors in the kidney of ApoE^–/– mice fed with HFD. (A) Representative immunoblotting of fibronectin, α‑SMA, collagen I, collagen III, collagen IV, TGF‑β1, and smad3 levels in the protein extractions from kidney cortex of normal and HFD fed ApoE^–/– mice treated with vehicle, SKF96365, Ad‑GFP–shRNA, or Ad‑Orai1–shRNA. (B) Quantitative analysis of profibrotic factor levels normalized to GADPH. Values are mean±SEM, n=6 in each group. *P≤0.05 or **P≤0.01 versus normal diet; ^#P≤0.05 or ^#P≤0.01 versus HFD+Ad‑GFP–shRNA; ^†P≤0.05 or ^††P≤0.01 versus HFD+vehicle.