Figure 7.

Inhibition of Orai1 Ca²⁺ channel by shRNA or SKF96365 prevented increased protein abundance of profibrotic factors in the kidney of mice with UUO. Representative immunoblotting of fibronectin, α‑SMA, TGF‑β1, and Orai1 levels in the protein extractions from kidney cortex of sham-operated mice and UUO mice treated with (A, B) Ad‑GFP–shRNA or Ad‑Orai1–shRNA; (C, D) vehicle PBS or SKF96365. Values are mean±SEM, n=6 in each group. **P≤0.01 versus sham; ^##P≤0.01 versus UUO7d‑G or UUO7d‑V. Sham-G, sham-O, sham-V, sham-S: sham-operated mice treated with Ad‑GFP–shRNA, Ad‑Orai1–shRNA, vehicle PBS, or SKF96365. UUO7d-G, UUO7d-O, UUO7d-V, UUO7d-S: 7-day UUO mice treated with Ad‑GFP–shRNA, Ad‑Orai1–shRNA, vehicle PBS, or SKF96365. UUO14d‑G, UUO14d‑O, UUO14d‑V, UUO14d‑S: 14-day UUO mice treated with Ad‑GFP–shRNA, Ad‑Orai1–shRNA, vehicle PBS, or SKF96365.