Table 3.

Proteins more and less abundant in uEVs of patients with ADPKD

Group/Protein	Group 1 (versus Healthy)a	Group 2 (versus Healthy)a	Group 2 (versus CKD)a
More abundant
Plakins
Desmoplakin	4.4	NC	NC
Envoplakinb,c	12.7	2.2	NC
Periplakinb	10.8	2.0	NC
Complement
Complement C3b	2.5	13.8	7.4
Complement C5	3.9	NC	74.8
Complement C4-B	3.1	5.9	4.3
Complement C9b	7.5	10.2	5.8
Complement factor B	13.7	20.3	8.5
Complement C1q subcomponent subunit A	2.0	NC	NC
Glycoproteins
Protein tweety homolog 3c	2.2	2.1	2.5
Isoform 2 of solute carrier family 22 member 13c	5.4	2.2	N.C.
V-set domain-containing T cell activation inhibitor 1	3.1	2.45	3.09
Retinoic acid-induced protein 3c	6.6	2.15	3.45
Pigment epithelium-derived factor	6.0	5.23	8.27
Heparin cofactor 2	2.1	30.32	NC
Inter-α-trypsin inhibitor heavy chain H1	3.2	NC	21.69
Miscellaneous
Villin-1b,c	3.0	2.4	11.8
Tyrosine-protein phosphatase nonreceptor type 13c	2.6	2.36	NC
Soluble of catechol O-methyltransferasec	2.1	4.10	2.98
Protein crumbs homolog 3c	2.6	NC	NC
Aconitate hydratase, mitochondrialc	3.0	3.05	NC
Apolipoprotein A-IVc	2.6	4.30	2.82
Cysteine-rich C-terminal protein 1c	15.8	13.05	NC
Glycogen phosphorylase, brain form	4.1	2.03	NC
Angiotensinogen	10.5	11.70	9.95
Calpain-5c	2.3	2.1	9.0
EH domain-containing protein 4	2.6	2.2	15.1
γ-synuclein	2.2	3.4	NC
Prothrombin	6.0	3.96	2.82
Plasminogen	23.6	4.84	2.77
Less abundant
Annexin A2	0.12	0.41	0.49
Contactin-1	0.43	0.26	0.14
Syndecan-4c	0.40	0.08	0.19
Granulins OS	0.34	0.50	0.23

NC, not calculated; EH, EPS15 homology.

^aMean ion intensities were compared and are reported as ratios. No ratio was calculated (NC) if the protein was identified in patients with ADPKD but was absent in the healthy persons or patients with non-ADPKD CKD (or identified at very low levels).

^bThese proteins were selected for confirmation and further characterization.

^cThese proteins were not identified in whole urine.