Figure 2.

Size distribution of APOL1 particles in EDTA plasma from subjects with or without the RRG, with or without ESRD as measured by size-exclusion chromatography on a Superdex 200 column. (A) Representative chromatograms of total cholesterol (dashed) or APOL1 (solid) in neat plasma separated by size-exclusion chromatography. The cholesterol peak eluting at 8 ml represents LDL and VLDL eluting in the void volume of the column, the heterogeneous cholesterol peak between 12 and 13 ml represents HDL. A fraction of APOL1 consistently elutes with VLDL/LDL and represents IgM-bound APOL1. The remaining APOL1 elutes largely between the HDL peak and the void volume suggesting a mol wt greater than the bulk of the HDL pool. Traces from healthy subjects and subjects with ESRD with or without the RRG are shown. (B) APOL1 levels in the normalized high, medium and low mol wt pools in five subjects without the RRG or seven subjects with the RRG are shown. Differences between low- and high-risk genotypes were tested by t test. No statistically significant changes (P<0.05) were noted.