Figure 1.

Generation and characterization of mice lacking TLR5 in the liver. (A) Analysis of albumin mRNA expression by quantitative RT-PCR in multiple organs of WT mice. (B–F) Analysis of TLR5 mRNA expression by quantitative RT-PCR in the (B) liver, (C) lung, (D) colon, (E) spleen, and (F) kidney of WT, TLR5^fl/fl, and TLR5^ΔHep mice. Results are expressed as relative values compared with the WT group, defined as 1. (G–L) Mice were injected intraperitoneally with 20 μg of purified flagellin (+) or vehicle (200 μL of sterile PBS [-]). Thirty minutes later, serum and organs were isolated. (G and H) Analysis of CXCL1 mRNA expression by quantitative RT-PCR in (G) liver and (H) colonic mucosa of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (I) Analysis of CXCL1 protein expression level by enzyme-linked immunosorbent assay in sera of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (J and K) Analysis of IL6 mRNA expression by quantitative RT-PCR in (J) liver and (K) colonic mucosa of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (L) Analysis of IL6 protein expression level by enzyme-linked immunosorbent assay in the serum of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (M and N) WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice were injected intraperitoneally with 20 μg of purified flagellin (+) or vehicle (200 μL of sterile PBS [-]), and serum were collected 120 minutes after. (M) Analysis of CXCL1 protein expression level by enzyme-linked immunosorbent assay in the serum of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (N) Analysis of IL6 protein expression level by enzyme-linked immunosorbent assay in the serum of WT, TLR5KO, TLR5^fl/fl, and TLR5^ΔHep mice. (B–N) Points are from individual mice, with bars representing means ± SEM. (A) Data are the means ± SEM. N = 2–6, except for panels I and L, with N = 5–15. Significance was determined by the Student t test. *P < .05.