Figure 1.

Chemical structure of PS VII and the effect of PS VII on U2OS osteosarcoma cell proliferation. (A) Chemical structure of PS VII. (B) Viability of U2OS cells was examined by MTT assay following 24 h treatment with 0, 0.25, 0.5, 1, 2.5 and 5 μM PS VII. (C) Viability of U2OS cells was examined by MTT assay following 0, 3, 6, 12, 24 and 48 h treatment with 0 (control), 1 and 2.5 μM PS VII. Values are presented as the mean ± standard deviation from three independent experiments. ^*P<0.05, ^**P<0.01 vs. control. PS VII, Paris saponin VII; MTT, 3-(4,5-dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide.