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. 2016 Aug 24;14(4):3595–3601. doi: 10.3892/mmr.2016.5667

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Expression levels of Ngn1 and BMP4 protein in NSCs following transfection with recombinant plasmid for 48 h. (A) Detection of Ngn1 and BMP4 protein levels by western blot analysis. β-actin was the internal standard. (B) Ngn1β-actin and BMP4β-actin protein optical density ratios were analyzed in each group. ^**P<0.01 vs. CON group; ^##P<0.01 vs. NC+N group; ^§§P<0.01 vs. NC+HIBD group. Ngn1, neurogenin 1; BMP4, bone morpho-genetic protein 4; NSC, neural stem cell; CON, blank control group; NC, negative control plasmid-transfected cells; N, normal brain tissue; HIBD, hypoxic-ischemic brain damage tissue; siNSC, β-catenin small interfering RNA-transfected cell.