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. 2016 Aug 8;14(4):3134–3144. doi: 10.3892/mmr.2016.5598

Figure 1.

Figure 1

Rapid generation of induced-resembled neuronal cells from A375 cells. (A and B) A375 melanoma cells infected by the empty viral vector with enhanced green fluorescent protein. The infection rate reached >95% with an MOI=20. The designated concentrations were derived as follows: MOI=1, 1×108 TU/ml of lentivirus + 0.1 µl lentivirus solution; MOI=10, 1×108 TU/ml of lentivirus + 1 µl lentivirus solution; MOI=100, 1×108 TU/ml of lentivirus + 1 µl lentivirus solution). The A375 cells were readily infected by the lentivirus. (A) Green fluorescence imaging was used to identify individual cells. (B) The same field was in white. (C and D) Following screening using puromycin, the percentage of cells infected by myelin transcription factor 1, which remained of the original group was ~65%, and the majority of cells exhibited axon-like changes. Magnification, ×200. MOI, multiplicity of infection. (C) The cells prior to screening. (D) The cells following screening.