Figure 3.

Importance of transduced factors in the reprogramming procedure. Quantification of Tuj1-positive cells with the indicated factor combinations of the brain protein 2 (B), achaete-scute homolog 1 (A), myelin transcription factor 1 (M) and neuronal differentiation factor 1 (N) genes 7 days following infection. Data are presented as the mean ± standard deviation of three randomly selected images (AxioCam), captured with a 200× objective on a Zeiss Axio Observer microscope. The total quantity of virus was constant between the different factor combinations, and the same numbers of A375 cells were seeded in each condition prior to infection. ^*P<0.05. Tuj1, tubulin β3.