Figure 4.

h-BDNF improves complex morphologies of irNCs. (A) At 1 week post-empty virus transduction, the cells in the five-factor and four-factor-infected cells were stained with Tuj1. (B) At 2 weeks post-empty virus transduction, the ABMN + h-BDNF and ABMN irNCs exhibited neuronal morphologies and expressed Tuj1. (C) Immunofluorescence images of the control, five-factor and four-factor groups on day 21. The (a) panels demonstrate the empty-virus group; the (b) panels demonstrate the five-factor group; and the (c) panels demonstrate the four-factor group. All images were captured under a microscope (magnification, ×200). irNCs, induced resembled neuronal cells; h-BDNF, human brain-derived neurotrophic factor; Tuj1, tubulin β3.